Difference between revisions of "Part:BBa K3482007"
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− | The His tag has been used to purify the azurin secreted outside of the E.coli | + | The His tag has been used to purify the azurin secreted outside of the E.coli Nissle 1917 cells. |
Revision as of 16:23, 23 October 2020
8x Histidine tag for protein purification
This 8x Histidine tag can be used to purify a protein. The tag has to be fused with his protein to be used in a purification.
Usage and Biology
The His tag has been used to purify the azurin secreted outside of the E.coli Nissle 1917 cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]