Difference between revisions of "Part:BBa K3482007"

(Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
  
The His tag has been used to purify the azurin secreted outside of the E.coli nissle 1917 cells.  
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The His tag has been used to purify the azurin secreted outside of the E.coli Nissle 1917 cells.  
  
  

Revision as of 16:23, 23 October 2020


8x Histidine tag for protein purification

This 8x Histidine tag can be used to purify a protein. The tag has to be fused with his protein to be used in a purification.


Usage and Biology

The His tag has been used to purify the azurin secreted outside of the E.coli Nissle 1917 cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]