Difference between revisions of "Part:BBa K3365054"

 
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<partinfo>BBa_K3365054 short</partinfo>
 
<partinfo>BBa_K3365054 short</partinfo>
  
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eGFP with double terminator
+
This part is used as a reporter for detecting whether dCas9-ωis off-target. We integrated this part downstream of transcription activating unit with lure sequence. If dCas9-ω is binding to lure sequence, this expression of this part will activate and the cell then emit green fluorescence, allowing us to know that dCas9-ωis off-target. As we plan to put the potential off-target site one by one, we added a terminator to eGFP so that the bound dCas9-ωcan only activating the expression downstream of its binding site.
  
 
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Revision as of 01:19, 25 October 2020


enhanced GFP with double terminator

This part is used as a reporter for detecting whether dCas9-ωis off-target. We integrated this part downstream of transcription activating unit with lure sequence. If dCas9-ω is binding to lure sequence, this expression of this part will activate and the cell then emit green fluorescence, allowing us to know that dCas9-ωis off-target. As we plan to put the potential off-target site one by one, we added a terminator to eGFP so that the bound dCas9-ωcan only activating the expression downstream of its binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
    Illegal XhoI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]