Difference between revisions of "Part:BBa K3657044"
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| − | + | Single-guide RNA for use with the catalytic core of a Tetrahymena thermophila trans-splicing ribozyme. | |
| + | Splicing results in the replacement of the 3' intron of the target sequence with mScarlet. | ||
| + | For more details see the design section of this part. | ||
| + | |||
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Latest revision as of 03:40, 28 October 2020
Split-Tetrahymena TransTransSplicing Guide with mScarlet
Single-guide RNA for use with the catalytic core of a Tetrahymena thermophila trans-splicing ribozyme. Splicing results in the replacement of the 3' intron of the target sequence with mScarlet. For more details see the design section of this part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 688
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]