Difference between revisions of "Part:BBa K2560001:Experience"
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===Applications of BBa_K2560001=== | ===Applications of BBa_K2560001=== | ||
| − | In our project we used this as a vector for using Golden Gate cloning to build our construct. The part was synthesized with added RFC10 prefix and suffix and then ligated to pSB1C3 according to the RFC10 standard. The vector plasmid was then purified and used in Golden Gate cloning. The new constructs made using this vector were then transformed into TOP10 cells which were grown on LB agar plates. The red fluorescence dropout colonies were easy to see by visual inspection but required over 24 hours after transformation for the RFP to be visible to the naked eye. The plates could be put in cold storage (4 °C) after 16 hours without any noticeable delay of the colonies becoming red | + | In our project we used this as a vector for using Golden Gate cloning to build our construct. The part was synthesized with added RFC10 prefix and suffix and then ligated to pSB1C3 according to the RFC10 standard. The vector plasmid was then purified and used in Golden Gate cloning. The new constructs made using this vector were then transformed into TOP10 cells which were grown on LB agar plates. The red fluorescence dropout colonies were easy to see by visual inspection but required over 24 hours after transformation for the RFP to be visible to the naked eye. The plates could be put in cold storage (4 °C) after 16 hours without any noticeable delay of the colonies becoming red [Aalto-Helsinki 2020]. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 06:13, 22 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2560001
In our project we used this as a vector for using Golden Gate cloning to build our construct. The part was synthesized with added RFC10 prefix and suffix and then ligated to pSB1C3 according to the RFC10 standard. The vector plasmid was then purified and used in Golden Gate cloning. The new constructs made using this vector were then transformed into TOP10 cells which were grown on LB agar plates. The red fluorescence dropout colonies were easy to see by visual inspection but required over 24 hours after transformation for the RFP to be visible to the naked eye. The plates could be put in cold storage (4 °C) after 16 hours without any noticeable delay of the colonies becoming red [Aalto-Helsinki 2020].
User Reviews
UNIQae6b0f916bcba984-partinfo-00000000-QINU UNIQae6b0f916bcba984-partinfo-00000001-QINU