Difference between revisions of "Part:BBa K3447006"

 
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<partinfo>BBa_K3447006 short</partinfo>
 
<partinfo>BBa_K3447006 short</partinfo>
  
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FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
 
  
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FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.<br><br>
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===Usage and Biology===
 
===Usage and Biology===
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We initiated the expression of downstream sfGFP BBa_K2541400 and repressor protein CI BBa_C0051 by connecting the chromogenic group (链接) of the blue light photosensitive system upstream of fixK2 promoters.<br>
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* Connect sfGFP gene downstream of fixK2 promoter: 链接<br>
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* Connect repressor proteins CI downstream of fixK2 promoter: 链接<br><br>
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==<b>Design</b>==
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===Design Notes===
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We added some synonymous mutations to avoid part rules.
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===Source===
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We found this sequence data in the previous iGEM teams (UCAS-China 2018) and in GenBank.<br><br>
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===References===
  
 
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Revision as of 05:51, 22 October 2020


xynD, which produces xylanase


FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.

Usage and Biology

We initiated the expression of downstream sfGFP BBa_K2541400 and repressor protein CI BBa_C0051 by connecting the chromogenic group (链接) of the blue light photosensitive system upstream of fixK2 promoters.

  • Connect sfGFP gene downstream of fixK2 promoter: 链接
  • Connect repressor proteins CI downstream of fixK2 promoter: 链接


Design

Design Notes

We added some synonymous mutations to avoid part rules.


Source

We found this sequence data in the previous iGEM teams (UCAS-China 2018) and in GenBank.

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]