Difference between revisions of "Part:BBa K3335000"

Revision as of 05:08, 22 October 2020

We use KIBRA to control exosome secretion

KIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion. Rab27a is stabilized by interacting with KIBRA, which prevents ubiquitination and degradation via the ubiquitin-proteasome pathway. KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a[1].

In our experiment, we used KIBRA to promote cell release of exosomes containing siRNA, thus increasing the efficiency of cell production and release of exosomes and improving the killing of tumor cells.

[1]Lin Song et al,.KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.Nature Communication,2019.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 335
Illegal BglII site found at 2999
Illegal BamHI site found at 246
Illegal BamHI site found at 442
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 435
Illegal BsaI site found at 2598
Illegal BsaI.rc site found at 1920
Illegal BsaI.rc site found at 2644

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 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-This year we want to use liver cells as a concept to treat lung cancer by overexpressing exosomes containing siRNA.
-In our interview with the professor, we learned that exosome production is likely to be inefficient, so we found the KIBRA protein.KIBRA as an adaptor - like protein that stabilizes Rab27a, which in turn controls exosome secretion.
-<img src="https://2020.igem.org/wiki/images/e/e8/T--NJU-China--KIBRA_mRNA_overexpression_in_HEK293T.jpg"/>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>