Difference between revisions of "Part:BBa K3657035"
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<partinfo>BBa_K3657035 short</partinfo> | <partinfo>BBa_K3657035 short</partinfo> | ||
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+ | This is a fusion protein containing AUAGAU binding Pumby Protein (<a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>) and GFP (<a href="https://parts.igem.org/Part:BBa_E0040">BBa_E0040</a>). | ||
+ | </p> | ||
+ | </html> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <html> | ||
+ | <p>AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>). Pumby Proteins can be designed to bind a specific RNA sequence.</p> | ||
+ | <p>GFP is a green fluorescent protein</p> | ||
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+ | </html> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3657035 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3657035 SequenceAndFeatures</partinfo> |
Revision as of 02:07, 28 October 2020
RNA AUAGAU binding Pumby Protein coupled with GFP
This is a fusion protein containing AUAGAU binding Pumby Protein (BBa_K3657004) and GFP (BBa_E0040).
Usage and Biology
AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(BBa_K3657015). Pumby Proteins can be designed to bind a specific RNA sequence.
GFP is a green fluorescent protein
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 184
Illegal XhoI site found at 224
Illegal XhoI site found at 449
Illegal XhoI site found at 634 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1337