Difference between revisions of "Part:BBa K3506011"

Revision as of 15:12, 27 October 2020

GAL1 promoter

It is the promoter of GAL1 gene, which codes galactokinase. For our project，we use it to express GFP fused with the first 124 amino acid of Clb2，then release the induction，if the fluorescence disappears，it can be proved that the first 124 amino acid of Clb2 have the ability to help proteins degrade.

Biology and Usage

It can be generally induced for gene expression by galactose and inhibited by glucose [1][2], making it a highly efficient inducible promoter for expression of foreign protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 70
1000
COMPATIBLE WITH RFC[1000]

Reference

[1] Johnston M. (1987). A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiological reviews, 51(4), 458–476. [2]Weinhandl, K., Winkler, M., Glieder, A., & Camattari, A. (2014). Carbon source dependent promoters in yeasts. Microbial cell factories, 13, 5. https://doi.org/10.1186/1475-2859-13-5

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 <partinfo>BBa_K3506011 short</partinfo>
-It is the promoter of GAL1 gene, which codes galactokinase. For our project&#65292;we use it to express GFP fused with the first 124 amino acid of Clb2&#65292;then release the induction&#65292;if the fluorescence disappears&#65292;it can be proved that the first 124 amino acid of Clb2 have the ability to help proteins degrade.
+It is the promoter of <i>GAL1</i> gene, which codes galactokinase. For our project&#65292;we use it to express GFP fused with the first 124 amino acid of Clb2&#65292;then release the induction&#65292;if the fluorescence disappears&#65292;it can be proved that the first 124 amino acid of Clb2 have the ability to help proteins degrade.
 <font size="4"><b>Biology and Usage</b></font>