Difference between revisions of "Part:BBa K3506009"

 
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This part consists of two parts: CLB2 promoter and Cas9. Combining these two parts enables the CLB2 promoter to control the expression of Cas9, so as to achieve the goal of making the expression level of Cas9 change with the cell cycle. Finally, Cas9 expression began in the S phase and reached its peak in the G2/M phase.
 
This part consists of two parts: CLB2 promoter and Cas9. Combining these two parts enables the CLB2 promoter to control the expression of Cas9, so as to achieve the goal of making the expression level of Cas9 change with the cell cycle. Finally, Cas9 expression began in the S phase and reached its peak in the G2/M phase.
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<b><font size="3">Usage</font></b>
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This part can be used to control Cas9 expression level with yeast cell cycle. If you want to control the expression of Cas9 during cell division, you can place this part on the yeast plasmid.
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Revision as of 05:29, 21 October 2020


The cassette of periodic expression of Cas9

This part consists of two parts: CLB2 promoter and Cas9. Combining these two parts enables the CLB2 promoter to control the expression of Cas9, so as to achieve the goal of making the expression level of Cas9 change with the cell cycle. Finally, Cas9 expression began in the S phase and reached its peak in the G2/M phase.

Usage

This part can be used to control Cas9 expression level with yeast cell cycle. If you want to control the expression of Cas9 during cell division, you can place this part on the yeast plasmid.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 812
    Illegal BglII site found at 1607
    Illegal BamHI site found at 1901
    Illegal XhoI site found at 203
    Illegal XhoI site found at 2407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1639
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 730
    Illegal BsaI.rc site found at 1972
    Illegal BsaI.rc site found at 3328
    Illegal BsaI.rc site found at 3751
    Illegal BsaI.rc site found at 3763
    Illegal BsaI.rc site found at 4630
    Illegal SapI.rc site found at 3435
    Illegal SapI.rc site found at 4017
    Illegal SapI.rc site found at 4032