Difference between revisions of "Part:BBa K3328027"
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===Design=== | ===Design=== | ||
OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation. | OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation. | ||
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| + | https://2020.igem.org/wiki/images/thumb/b/b3/T--OUC-China--design_lunbo_or.jpg/799px-T--OUC-China--design_lunbo_or.jpg | ||
===Result=== | ===Result=== | ||
Revision as of 12:45, 22 October 2020
switch of OR gate
realize the boolean calculation of OR
Design
OR gate composed of two switch RNA hairpins. Each switch module has an input RNA recognition site and its own RBS and start codon. Input RNA binding unwinds the corresponding switch stem to activate translation.
Result
The left figure shows that when INPUT A=0, INPUT B=0, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 819