Difference between revisions of "Part:BBa K3328008"

Revision as of 17:06, 20 October 2020

ON-switch

an ON-switch to regulate the expression of the downstream gene

Design

Toehold switch is composed of cis-acting element RNA hairpins and trans-acting factor trigger RNA. The binding of a trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation.

Result

Compared with the blank control (IPTG=0 M, aTc=0 mg/ml), the fluorescence of GFP was low when only the promoter before toehold sequence was turned on (IPTG=0.1 M, aTc=0 mg/ml). This indicates that the toehold has the advantage of low leakage. When the trigger was expressed (IPTG=0.1 M, aTc=0.25 mg/ml), it showed a high GFP fluorescence of up to 32.3-fold due to the destruction of toehold hairpin structure.

Usage and Biology

Sequence and Features