Difference between revisions of "Part:BBa K3328015"

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realize the boolean calculation of XOR
 
realize the boolean calculation of XOR
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===Design===
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The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.
  
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===Result===
 
https://2020.igem.org/wiki/images/thumb/1/14/T--OUC-China--part_XOR2.jpg/800px-T--OUC-China--part_XOR2.jpg
 
https://2020.igem.org/wiki/images/thumb/1/14/T--OUC-China--part_XOR2.jpg/800px-T--OUC-China--part_XOR2.jpg
 
The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
 
The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
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===User Reviews===
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 16:56, 20 October 2020


switch of XOR gate (XOR2)

realize the boolean calculation of XOR

Design

The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.

Result

800px-T--OUC-China--part_XOR2.jpg The left figure shows that when INPUT A and INPUT B are both 0 or 1, the fluorescence of GFP is low. And the fluorescence intensity of GFP was high in the other two groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.

User Reviews

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 746