Difference between revisions of "Part:BBa K3582026:Design"
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Latest revision as of 18:12, 23 October 2020
Inhibitory sequence 1 of PfEMP1 - ICAM1 interaction grafted into a cyclotide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 112
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 143
Illegal BsaI.rc site found at 109
Design Notes
- 1] RFC10 criteria satisfied
- 2] Strep Ⅱ tag gene inserted in loop 3
- 3] Codon optimized with E-coli K-12 strain
- 4] Inhibitor 1 grafted in loop 6
- 5] C and N intein added for circularization
Source
Reverse translation of amino acid sequence