Difference between revisions of "Part:BBa K3505034"

Revision as of 14:18, 26 October 2020

pAndersonJ23115:tetO:RBS-lacI-terminator

Usage and Biology

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha vector BBa_K3505009 and has overhangs compatible for Golden Braid cloning.

Figure 2. The level A module of Tet-Off system : AndersonJ23115:TetO:RBS-LacI-Double terminator

Verification of Cloning

Fig.1:(U=Uncut C=Cut) Restriction digestion of TL: AndersonJ23115:Tet0-LacI-double terminator(C5-C8 ) with: HindIII + NheI (C5-C18) , Expected bands : 2573+891+427,Positive result : C5,C6

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3505034 short</partinfo>
-long
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Design Notes===
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha vector <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for Golden Braid cloning.
+[[Image:T--Thessaly--teO-LacI-PHOT.png|900px|thumb|none|<I><b>Figure 2.</b> The level A module of Tet-Off system : AndersonJ23115:TetO:RBS-LacI-Double terminator </i>]]
+===Verification of Cloning===
+[[File:T--Thessaly--tetO-LacI-digestionpng.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction digestion of TL: AndersonJ23115:Tet0-LacI-double terminator(C5-C8 ) with: HindIII + NheI (C5-C18) , Expected bands : 2573+891+427,Positive result : C5,C6</i>]]
+===Sequence and Features===
 <partinfo>BBa_K3505034 SequenceAndFeatures</partinfo>