Difference between revisions of "Part:BBa K3505026"

 
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3505026 SequenceAndFeatures</partinfo>
 
  
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===Design Notes===
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for Golden Braid cloning.
  
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===Functional Parameters===
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[[Image:T--Thessaly--B-ARRESTIN-TEV-PHOT.png|900px|thumb|none|<I><b>Figure 2.</b> The level B module of GPCR-Tango module : ParaBAD:RBS:B-arrestin -TEV protease -Double Terminator</i>]]
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<partinfo>BBa_K3505026 parameters</partinfo>
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===Sequence and Features===
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<partinfo>BBa_K3505026 SequenceAndFeatures</partinfo>

Revision as of 16:57, 26 October 2020


ParaBAD:RBS- β-arrestin2:TEVp:terminator

long


Usage and Biology

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector BBa_K3505009 and has overhangs compatible for Golden Braid cloning.


Figure 2. The level B module of GPCR-Tango module : ParaBAD:RBS:B-arrestin -TEV protease -Double Terminator


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1446
    Illegal BamHI site found at 1148
    Illegal XhoI site found at 1843
    Illegal XhoI site found at 2237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
    Illegal AgeI site found at 983
    Illegal AgeI site found at 1829
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 965
    Illegal SapI.rc site found at 2788
    Illegal SapI.rc site found at 3136