Difference between revisions of "Part:BBa K3328025"

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realize the boolean calculation of NIMPLY
 
realize the boolean calculation of NIMPLY
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===Design===
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In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.
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===Result===
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https://2020.igem.org/wiki/images/thumb/1/1f/T--OUC-China--part_NIMPLY2.jpg/800px-T--OUC-China--part_NIMPLY2.jpg
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The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:25, 20 October 2020


switch of NIMPLY gate (NIMPLY2)

realize the boolean calculation of NIMPLY

Design

In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.

Result

800px-T--OUC-China--part_NIMPLY2.jpg

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 778