Difference between revisions of "Part:BBa K3505016"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | < | + | ===Design Notes=== |
− | < | + | The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 <b>BBa_K3505007 </b> and has overhangs compatible for Golden Braid cloning. |
+ | The CDS has position A1-B2 | ||
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+ | <p style="text-align: center;"> | ||
+ | [[Image:T--Thessaly--GB-GGAG-CCAT.jpeg |900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the Golden Braid Grammar.</i>]] | ||
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+ | [[Image:T--Thessaly--paracBAD-snap.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- araC:ParaBAD (illustration from SnapGene)</i>]] | ||
+ | |||
+ | ===Verification of Cloning=== | ||
+ | [[File:T--Thessaly--araBAD-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction Digestion of araC:ParaBAD with EcoRI+ PstI ,Expected bands: 2029+1996, Positive result : YES </i>]] | ||
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+ | ===Sequence and Features=== | ||
<partinfo>BBa_K3505016 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3505016 SequenceAndFeatures</partinfo> | ||
Revision as of 14:49, 26 October 2020
araC-ParaBAD:RBS GB compatible with A1-B1
long
Usage and Biology
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. The CDS has position A1-B2
Verification of Cloning
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1148
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 983
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 965