Difference between revisions of "Part:BBa K3611007"
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+ | __NOTOC_7 | ||
+ | <partinfo>BBa_K3611000 short</partinfo> | ||
+ | |||
+ | This is the coding gene of egfp fusing AAV protein degradation tag.To reduce the sensor basal background, we use protease-based post-transcriptional degradation regulation. By adding a protein degradation tag (AAV) to the reporter protein EGFP, the output basal expression was reduced, also the sensor’s soutput level. | ||
+ | |||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3611007 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3611007 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 13:22, 20 October 2020
__NOTOC_7 The coding gene of ACE2-PmrB recombinant protein.
This is the coding gene of egfp fusing AAV protein degradation tag.To reduce the sensor basal background, we use protease-based post-transcriptional degradation regulation. By adding a protein degradation tag (AAV) to the reporter protein EGFP, the output basal expression was reduced, also the sensor’s soutput level.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644