Difference between revisions of "Part:BBa K3328017:Design"

Revision as of 13:35, 20 October 2020

switch of IMPLY gate (IMPLY1)

Assembly Compatibility:

Realize of the boolean calculation of IMPLY

synthesize from company

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 ===Design Notes===
-We combined the 3wj switch and toehold switch to realize the IMPLY Boolean calculation. When no trigger expressed, this logic gate just behaves like a 3wj switch. When trigger A expressed, the trigger will bind to the switch RNA. The binding of trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation [1]. When trigger B expressed, the trigger, trigger will bind to 3wj switch RNA. The resulting trigger–switch complex has a stable 3WJ structure that effectively sequesters the RBS and start codon within the loop and stem of the switch RNA, respectively, and strongly represses translation. When trigger A and B both expressed, the binding of trigger RNA to the toehold sequence allow the RNA polymerase binds to the former RBS and break open 3WJ stable hairpin.
+Realize of the boolean calculation of IMPLY
 ===Source===