Difference between revisions of "Part:BBa K3482018"
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<partinfo>BBa_K3482018 short</partinfo>
 
<partinfo>BBa_K3482018 short</partinfo>
  
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MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG
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MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor
  
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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Colicin E2 is an endonuclease that cut on both single and double-stranded DNA. It has no specific cutting site. Colicin toxin are toxins that can be produced by E.coli and closely related bacteria.
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We will use this part in a Kill-switch with the IM2 immunity protein to Colicin E2.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3482018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3482018 SequenceAndFeatures</partinfo>

Revision as of 14:35, 20 October 2020


IPTG and heat-repressible miniColicin toxin

MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor


Usage and Biology

Colicin E2 is an endonuclease that cut on both single and double-stranded DNA. It has no specific cutting site. Colicin toxin are toxins that can be produced by E.coli and closely related bacteria.

We will use this part in a Kill-switch with the IM2 immunity protein to Colicin E2.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
    Illegal AgeI site found at 155
  • 1000
    COMPATIBLE WITH RFC[1000]