Difference between revisions of "Part:BBa K3468072:Experience"

 
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===Applications of BBa_K3468072===
 
===Applications of BBa_K3468072===
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The reduction of DDG was predicted by FoldX. PyOML was used for visual screening. it formed hydrogen bonds with 186-HIS and 190-ASN. after mutation to ASN, it formed hydrogen bonds with 190-ASN, which stabilized α5-β7loop and was beneficial to improve the thermal stability of PETase.
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[[File:S188N.png|400px|thumb|left|Fig.1 S188N in PyMOL]]
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===User Reviews===
 
===User Reviews===

Revision as of 09:45, 25 October 2020


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3468072

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening. it formed hydrogen bonds with 186-HIS and 190-ASN. after mutation to ASN, it formed hydrogen bonds with 190-ASN, which stabilized α5-β7loop and was beneficial to improve the thermal stability of PETase.

Fig.1 S188N in PyMOL


User Reviews

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