Difference between revisions of "Part:BBa K112245"

 
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__NOTOC__
 
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<partinfo>BBa_K112245 short</partinfo>
 
<partinfo>BBa_K112245 short</partinfo>
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This part was inserted into the K112995 plasmid between ccdB and the attR2 site.
 
This part was inserted into the K112995 plasmid between ccdB and the attR2 site.
  
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Performing Gateway ''in vivo'' was tested using this part and an RFP part flanked by att sites in an entry vector. Three trials showed very high efficiency(transformations using miniprepped material gave thousands of red colonies), and low co-transformation rate(less than 1%). For more information about this part, visit our [http://2008.igem.org/Team:UC_Berkeley/LysisDevice Wiki!]
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This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:
 
This part is in BBb Format.  It is flanked by BamHI and BglII sites instead of XbaI and SpeI.  More information about the BBb Format is available at:
  

Revision as of 09:16, 6 November 2008

{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid

This part was inserted into the K112995 plasmid between ccdB and the attR2 site.

Performing Gateway in vivo was tested using this part and an RFP part flanked by att sites in an entry vector. Three trials showed very high efficiency(transformations using miniprepped material gave thousands of red colonies), and low co-transformation rate(less than 1%). For more information about this part, visit our [http://2008.igem.org/Team:UC_Berkeley/LysisDevice Wiki!]

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3242
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4084
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
    Illegal NotI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 212
    Illegal XhoI site found at 5265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3242
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3242
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
    Illegal AgeI site found at 3674
    Illegal AgeI site found at 3998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 762
    Illegal BsaI.rc site found at 4366