Difference between revisions of "Part:BBa K3482004"
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===Usage=== | ===Usage=== | ||
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| + | We used that part to test if the expression of the toxin work in our E.coli nissle 1917 strain and if it was sufficient do provoc cell death | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 14:44, 20 October 2020
IPTG-inducible miniColicin toxin
This part allows for IPTG inducible expression of the DNAase miniColicin in E. coli. The degradation of DNA , which leads to cell death, can therefore be induced by IPTG.
Biology
Usage
We used that part to test if the expression of the toxin work in our E.coli nissle 1917 strain and if it was sufficient do provoc cell death
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 124
- 1000COMPATIBLE WITH RFC[1000]