Difference between revisions of "Part:BBa K3423001"

Revision as of 00:53, 20 October 2020

Anti-CRISPR (acr) promoter region

This Anti-CRISPR promoter is a constitutive promoter that can be inhibited by protein Anti-CRISPR-associated 1 (Stanley et al., 2019).

Protein anti-CRISPR-associated 1 (Aca1) is expressed by T7 promoter, Aca1 will subsequently inhibit the anti-CRISPR promoter, so the gene expression downstream anti-CRISPR will be inhibited until Navitoclax (a compound that can inhibit Aca1 found by SUSTech_Shenzhen) is added artificially. Without the inhibition from Aca1, the expression of genes downstream anti-CRISPR promoter will increase significantly. Therefore, protein expression can be regulated artificially.

We characterised the efficiency of this part of promoting a reporter gene in a protein regulation system, the reporter was sufficiently expressed for measurement, acr promoter successfully worked in our composite part BBa_K3423005.

Reference

Stanley, S. Y., Borges, A. L., Chen, K.-H., Swaney, D. L., Krogan, N. J., Bondy-Denomy, J., & Davidson, A. R. (2019). Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription. Cell, 178(6), 1452-1464.e1413. doi:10.1016/j.cell.2019.07.046

Sequence and Features