Difference between revisions of "Part:BBa K3351011"
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K3351011 parameters</partinfo>
 
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<partinfo>BBa_K3351011 short</partinfo>
 
<partinfo>BBa_K3351011 short</partinfo>
 
===Summary===
 
===Summary===
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[3] Kuersten, S. and Goodwin, E. B. (2003) The power of the 3′ UTR: translational control and development Nat. Rev. Genet. 4, 626– 637
 
[3] Kuersten, S. and Goodwin, E. B. (2003) The power of the 3′ UTR: translational control and development Nat. Rev. Genet. 4, 626– 637
 
[4] Yamanishi, M., Ito, Y., Kintaka, R., Imamura, C., Katahira, S., Ikeuchi, A., Moriya, H., & Matsuyama, T. (2013). A genome-wide activity assessment of terminator regions in saccharomyces cerevisiae provides a "terminatome" toolbox. ACS Synthetic Biology, 2(6), 337-347.
 
[4] Yamanishi, M., Ito, Y., Kintaka, R., Imamura, C., Katahira, S., Ikeuchi, A., Moriya, H., & Matsuyama, T. (2013). A genome-wide activity assessment of terminator regions in saccharomyces cerevisiae provides a "terminatome" toolbox. ACS Synthetic Biology, 2(6), 337-347.
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'''Secondary Structure'''
 
  
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[[Image:Mfold-K3351011-1.png]]
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===Usage and Biology===
  
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'''Measurement'''
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<span class='h3bb'>Sequence and Features</span>
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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<partinfo>BBa_K2922040 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2922040 parameters</partinfo>
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Revision as of 13:52, 19 October 2020


A T7 terminator of pet28a plasmid.

Summary

Terminator regions, which are located downstream of protein coding sequences, encode 3′ untranslated regions (3′-UTRs) of mRNA. Terminator regions are usually involved in two complex events: ″transcriptional termination″, which involves the cleavage of 3′-mRNA and poly(A) addition and ″post-transcriptional regulation″, in which the 3′-UTR determines the stability, translational efficiency, and localization of the mRNA. Sequence and structural cis-elements located in the 3′-UTR of mRNA transcripts interact with RNA-binding proteins (RBPs) to post-transcriptionally regulate various aspects of gene expression including mRNA localization, translation, and decay.


Reference

[1] Richard, P. and Manley, J. L. (2009) Transcription termination by nuclear RNA polymerases Genes Dev. 23, 1247– 1269 [2] Kuehner, J. N., Pearson, E. L., and Moore, C. (2011) Unravelling the means to an end: RNA polymerase II transcription termination Nat. Rev. Mol. Cell. Biol. 12, 283– 294 [3] Kuersten, S. and Goodwin, E. B. (2003) The power of the 3′ UTR: translational control and development Nat. Rev. Genet. 4, 626– 637 [4] Yamanishi, M., Ito, Y., Kintaka, R., Imamura, C., Katahira, S., Ikeuchi, A., Moriya, H., & Matsuyama, T. (2013). A genome-wide activity assessment of terminator regions in saccharomyces cerevisiae provides a "terminatome" toolbox. ACS Synthetic Biology, 2(6), 337-347.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]