Difference between revisions of "Part:BBa K3425001"

 
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<partinfo>BBa_K3425001 short</partinfo>
 
<partinfo>BBa_K3425001 short</partinfo>
  
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Description coming soon
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===iGEM Type IIS Vectors===
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====pEven Loop Vectors (for Level 2)====
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{{CSS/DNA_Table}}
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When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with <partinfo>BBa_J04455</partinfo>), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites.
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<html>
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<center>
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<table class="dnaTable">
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<tr>
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<th>Part number</th>
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<th>Name</th>
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<th>Loop Name</th>
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<th>Fusion Site 5'</th>
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<th>MTU</th>
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<th>Fusion Site 3'</th>
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</tr>
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<tr>
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<td></html>[[Part:BBa_K3425001|<partinfo>BBa_K3425001</partinfo>]]<html></td>
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<td>pSB3C11</td>
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<td>pEven1</td>
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<td class="dnaSeq rightAlign">GGAG</td>
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<td class="centerAlign partType" style="background-color: #00B050;">MTU 1</td>
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<td class="dnaSeq">TACT</td>
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</tr>
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<tr>
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<td></html>[[Part:BBa_K3425002|<partinfo>BBa_K3425002</partinfo>]]<html></td>
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<td>pSB3C12</td>
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<td>pEven2</td>
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<td class="dnaSeq rightAlign">TACT</td>
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<td class="centerAlign partType" style="background-color: #FFC001;">MTU 2</td>
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<td class="dnaSeq">AATG</td>
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</tr>
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<tr>
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<td></html>[[Part:BBa_K3425003|<partinfo>BBa_K3425003</partinfo>]]<html></td>
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<td>pSB3C13</td>
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<td>pEven3</td>
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<td class="dnaSeq rightAlign">AATG</td>
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<td class="centerAlign partType" style="background-color: #4472C4;">MTU 3</td>
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<td class="dnaSeq">GCTT</td>
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</tr>
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<tr>
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<td></html>[[Part:BBa_K3425004|<partinfo>BBa_K3425004</partinfo>]]<html></td>
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<td>pSB3C14</td>
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<td>pEven4</td>
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<td class="dnaSeq rightAlign"> GCTT </td>
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<td class="centerAlign partType" style="background-color: #FF0000;">MTU 4</td>
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<td class="dnaSeq">CGCT</td>
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</tr>
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</table>
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</center>
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</html>
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{{TypeIIS/References}}
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'''Note:''' This section was adapted from [[Part:pSB3C01|<partinfo>pSB3C01</partinfo>]] in order to collect all the information in one page.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:50, 20 October 2020


pSB3C11: Improved pEven1 Loop Vector based on pSB3C01


iGEM Type IIS Vectors

pEven Loop Vectors (for Level 2)

When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with BBa_J04455), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites.

Part number Name Loop Name Fusion Site 5' MTU Fusion Site 3'
BBa_K3425001 pSB3C11 pEven1 GGAG MTU 1 TACT
BBa_K3425002 pSB3C12 pEven2 TACT MTU 2 AATG
BBa_K3425003 pSB3C13 pEven3 AATG MTU 3 GCTT
BBa_K3425004 pSB3C14 pEven4 GCTT MTU 4 CGCT


The iGEM Type IIS assembly standard is based on MoClo and Loop

Note: This section was adapted from pSB3C01 in order to collect all the information in one page.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal NheI site found at 1399
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 6