Difference between revisions of "Part:BBa K3409012"

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Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007) and a double terminator (BBa_B0015). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.  
 
Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007) and a double terminator (BBa_B0015). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.  
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===Characterization===
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NOTE: In this section: BB1 = part BBa_K3409010  -  BB2 = part BBa_K3409011 – BB3 = part BBa_K3409012
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PCR AMPLIFICATION OF PARTS
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[[File: PCR_BB1_BB2_BB3.png|border|700px]]
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Once received, the synthetized fragments are resuspended and amplified via polymerase chain reaction (PCR) to obtain a higher quantity of DNA. Every PCR sample is verified by gel electrophoresis to analyze the PCR results. First, the appropriate bands for BioBrick 1 (1.5 kb) , BioBrick 2 (1.4 kb) and BioBrick 3 (2.4 kb) amplified with the primers for Hifi Assembly method, are present at their expected size (Figure 1A).
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Then, the appropriate bands for BioBrick 1 (1.5 kb), BioBrick 2 (1.4 kb) and BioBrick 3 (2.4 kb) amplified with the primers for BioBrick Assembly method, are present at their expected size on Figure 1B.
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Revision as of 21:03, 25 October 2020


Microcin PDI cluster gene for McpB

Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007) and a double terminator (BBa_B0015). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.

Characterization

NOTE: In this section: BB1 = part BBa_K3409010 - BB2 = part BBa_K3409011 – BB3 = part BBa_K3409012

PCR AMPLIFICATION OF PARTS

PCR BB1 BB2 BB3.png

Once received, the synthetized fragments are resuspended and amplified via polymerase chain reaction (PCR) to obtain a higher quantity of DNA. Every PCR sample is verified by gel electrophoresis to analyze the PCR results. First, the appropriate bands for BioBrick 1 (1.5 kb) , BioBrick 2 (1.4 kb) and BioBrick 3 (2.4 kb) amplified with the primers for Hifi Assembly method, are present at their expected size (Figure 1A). Then, the appropriate bands for BioBrick 1 (1.5 kb), BioBrick 2 (1.4 kb) and BioBrick 3 (2.4 kb) amplified with the primers for BioBrick Assembly method, are present at their expected size on Figure 1B.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]