Difference between revisions of "Part:BBa K3629013"
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===Design=== | ===Design=== | ||
− | The native signal peptide from <i> | + | The native signal peptide from <i>P. funiculosum</I> was removed so it would not interfere with the fused Lip2 secretion tag native to <i>Y. lipolytica.</i> |
===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 04:43, 19 October 2020
Modified PfCBHI expression construct with gibson homology sequences and 6X His tag
Usage and Biology
Design
The native signal peptide from P. funiculosum was removed so it would not interfere with the fused Lip2 secretion tag native to Y. lipolytica.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2725
Illegal EcoRI site found at 2546 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2546
Illegal NheI site found at 75
Illegal SpeI site found at 2726
Illegal PstI site found at 2740
Illegal NotI site found at 7
Illegal NotI site found at 2733 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2546
Illegal BglII site found at 1322
Illegal BamHI site found at 2631
Illegal XhoI site found at 87 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2726
Illegal EcoRI site found at 2546 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2546
Illegal XbaI site found at 16
Illegal SpeI site found at 2726
Illegal PstI site found at 2740
Illegal NgoMIV site found at 804 - 1000COMPATIBLE WITH RFC[1000]