Difference between revisions of "Part:BBa K3629017"
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===Design=== | ===Design=== | ||
− | The native signal peptide from <I>T. reesei</ | + | The native signal peptide from <I>T. reesei</I> was removed so it would not interfere with the fused XRP2 secretion tag native to <i>Y. lipolytica.</i> |
===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 04:45, 19 October 2020
T. reesei EGII expression construct with gibson homology sequences and FLAG tag
Usage and Biology
Design
The native signal peptide from T. reesei was removed so it would not interfere with the fused XRP2 secretion tag native to Y. lipolytica.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2835
Illegal EcoRI site found at 2698
Illegal SpeI site found at 749 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2698
Illegal NheI site found at 68
Illegal NheI site found at 810
Illegal SpeI site found at 749
Illegal SpeI site found at 2836
Illegal PstI site found at 2850
Illegal NotI site found at 7
Illegal NotI site found at 2843 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2698
Illegal BamHI site found at 2786
Illegal XhoI site found at 125 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2836
Illegal EcoRI site found at 2698
Illegal SpeI site found at 749 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2698
Illegal XbaI site found at 16
Illegal SpeI site found at 749
Illegal SpeI site found at 2836
Illegal PstI site found at 2850
Illegal NgoMIV site found at 1496
Illegal NgoMIV site found at 2030
Illegal NgoMIV site found at 2348 - 1000COMPATIBLE WITH RFC[1000]