Difference between revisions of "Part:BBa K3577000"
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<partinfo>BBa_K3577000 short</partinfo> | <partinfo>BBa_K3577000 short</partinfo> | ||
− | + | The function of this part is to produce toehold switch in vitro. It consists of T7 promoter, toehold switch (BBa_K3577001), mCherry and T7 terminator. | |
+ | Toehold switch is a special RNA hairpin structure, which contains trigger strand, ribosome binding site, translation start codon and a report gene. Our toehold switch sequence (BBa_K3577001) is based on an article called Toehold Switches: De-Novo-Designed Regulators of Gene Expression (Green A, et al., 2014) and the mCherry is added to the downstream of the RNA hairpin structure. | ||
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Revision as of 02:38, 19 October 2020
T7-toehold switch-mCherry
The function of this part is to produce toehold switch in vitro. It consists of T7 promoter, toehold switch (BBa_K3577001), mCherry and T7 terminator. Toehold switch is a special RNA hairpin structure, which contains trigger strand, ribosome binding site, translation start codon and a report gene. Our toehold switch sequence (BBa_K3577001) is based on an article called Toehold Switches: De-Novo-Designed Regulators of Gene Expression (Green A, et al., 2014) and the mCherry is added to the downstream of the RNA hairpin structure.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]