Difference between revisions of "Part:BBa K079039"
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The LexA repressor from Escherichia coli negatively regulates the transcription of about 20 different genes upon binding with variable affinity. Nature itself provide us a wide spectrum of operator sites with different activation thresholds. | The LexA repressor from Escherichia coli negatively regulates the transcription of about 20 different genes upon binding with variable affinity. Nature itself provide us a wide spectrum of operator sites with different activation thresholds. | ||
| − | [[Image:Lex Symmetrical.jpg |left| | + | [[Image:Lex Symmetrical.jpg |left|300px|thumbnail|LexA perfectly symmetrical sequence ]] |
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079039 K079039]: Lex A1 is a perfectly 2-fold symmetrical consensus sequence binding LexA more tightly than any natural occurring SOS promoter. [https://parts.igem.org/Part:BBa_K079039:Design Published data] show the details of this interaction but in vivo measurment of repression levels are still scarce | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079039 K079039]: Lex A1 is a perfectly 2-fold symmetrical consensus sequence binding LexA more tightly than any natural occurring SOS promoter. [https://parts.igem.org/Part:BBa_K079039:Design Published data] show the details of this interaction but in vivo measurment of repression levels are still scarce | ||
| − | [[Image:RecA.jpg |right| | + | [[Image:RecA.jpg |right|300px|thumbnail|RecA gene Operator Sequence ]] |
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079040 K079040]: Lex A2 is the wild type operator sequence controlling RecA protein expression. Being the first activated response gene in the LexA degradation driven [http://2008.igem.org/Team:Bologna/Results SOS Pathway] the respective operator have to be one of the least LexA affine natural sequence. | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079040 K079040]: Lex A2 is the wild type operator sequence controlling RecA protein expression. Being the first activated response gene in the LexA degradation driven [http://2008.igem.org/Team:Bologna/Results SOS Pathway] the respective operator have to be one of the least LexA affine natural sequence. | ||
Revision as of 18:03, 28 October 2008
LexA 1 - operator library member
This parts have been extracted from the LexA Operator Library.
The LexA repressor from Escherichia coli negatively regulates the transcription of about 20 different genes upon binding with variable affinity. Nature itself provide us a wide spectrum of operator sites with different activation thresholds.
K079039: Lex A1 is a perfectly 2-fold symmetrical consensus sequence binding LexA more tightly than any natural occurring SOS promoter. Published data show the details of this interaction but in vivo measurment of repression levels are still scarce
K079040: Lex A2 is the wild type operator sequence controlling RecA protein expression. Being the first activated response gene in the LexA degradation driven [http://2008.igem.org/Team:Bologna/Results SOS Pathway] the respective operator have to be one of the least LexA affine natural sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]