Difference between revisions of "Part:BBa K3487010"
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<partinfo>BBa_K3487010 short</partinfo>
 
<partinfo>BBa_K3487010 short</partinfo>
  
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===Description===
 
This part can be used directly as a suicide switch to prevent engineered bacteria from escaping into the environment, and is suitable for a variety of common engineered bacteria, such as BL21 or DH5&#945;.
 
This part can be used directly as a suicide switch to prevent engineered bacteria from escaping into the environment, and is suitable for a variety of common engineered bacteria, such as BL21 or DH5&#945;.
  
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K3487010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3487010 SequenceAndFeatures</partinfo>
  
 
===Reference===
 
===Reference===
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    Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.
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Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.
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    Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.
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<br>
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Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3487010 parameters</partinfo>
 
<partinfo>BBa_K3487010 parameters</partinfo>
 
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Revision as of 13:41, 18 October 2020


A kill switch regulated by lysine concentration

Description

This part can be used directly as a suicide switch to prevent engineered bacteria from escaping into the environment, and is suitable for a variety of common engineered bacteria, such as BL21 or DH5α.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 256

Reference

Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.
Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.