Difference between revisions of "Part:BBa K079036"

Revision as of 14:58, 28 October 2008

Tet O operator library member

These parts have been extracted from the TeT Operator Library.

Repression efficiency of Tet Operators Variants expressed as β-galactosidase percentage activity. 100% Being unrepressed

This subset of operator sequences has been chosen since it allows to cover a wide affinity and repression range

K079036:Tet O is the Wild Type operator sequence regulating Tetracycline resistance genes. The natural sequence exhibit a tigth repression given by an high Tet R bindig affinity
K079037:TetO-4C is a medium affinity operator obtained through a T-C mutation in the fourth nucleotide on both side of the symmetric consensus sequence. Disrupting this interaction the repression efficiency is about 12 time lower than the Wt
K079038: TetO-wt/4C5G is a double mutant that heavily compromises the interaction of TetR with the right half of the binding sequence. The resulting repression is quite weak, more than 40 times less stringent than in TetO

Sequence and Features