Difference between revisions of "Part:BBa K3629012"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | ===Design=== | |
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− | <partinfo> | + | ===Sequence and Features=== |
+ | <partinfo>BBa_K3629012 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K3629012 parameters</partinfo> |
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Revision as of 07:17, 18 October 2020
T. reesei CBHII expression construct with gibson homology sequences and FLAG tag
Usage and Biology
Design
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2737
Illegal EcoRI site found at 2561 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2561
Illegal NheI site found at 75
Illegal SpeI site found at 2738
Illegal PstI site found at 2752
Illegal NotI site found at 7
Illegal NotI site found at 2745 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 2561
Illegal BamHI site found at 2686
Illegal XhoI site found at 124
Illegal XhoI site found at 1198 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2738
Illegal EcoRI site found at 2561 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 2561
Illegal XbaI site found at 16
Illegal SpeI site found at 2738
Illegal PstI site found at 2752
Illegal NgoMIV site found at 1458 - 1000COMPATIBLE WITH RFC[1000]