Difference between revisions of "Part:BBa K3629012"

 
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__NOTOC__
 
__NOTOC__
<partinfo>BBa_K3629015 short</partinfo>
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<partinfo>BBa_K3629012 short</partinfo>
  
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===Usage and Biology===
 
===Usage and Biology===
  
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===Design===
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3629015 SequenceAndFeatures</partinfo>
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===Sequence and Features===
 +
<partinfo>BBa_K3629012 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
<partinfo>BBa_K3629015 parameters</partinfo>
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<partinfo>BBa_K3629012 parameters</partinfo>
 
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Revision as of 07:17, 18 October 2020


T. reesei CBHII expression construct with gibson homology sequences and FLAG tag


Usage and Biology

Design

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2737
    Illegal EcoRI site found at 2561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2561
    Illegal NheI site found at 75
    Illegal SpeI site found at 2738
    Illegal PstI site found at 2752
    Illegal NotI site found at 7
    Illegal NotI site found at 2745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2561
    Illegal BamHI site found at 2686
    Illegal XhoI site found at 124
    Illegal XhoI site found at 1198
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2738
    Illegal EcoRI site found at 2561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2561
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2738
    Illegal PstI site found at 2752
    Illegal NgoMIV site found at 1458
  • 1000
    COMPATIBLE WITH RFC[1000]