Difference between revisions of "Part:BBa K3352000"
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We obtained the amino acid sequence of the SplintR ligase protein found in Paramecium bursaria Chlorella virus 1. We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site. We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker). | We obtained the amino acid sequence of the SplintR ligase protein found in Paramecium bursaria Chlorella virus 1. We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site. We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker). | ||
Revision as of 15:50, 18 October 2020
SplintR Ligase with His-Tag and GS Linker Sequence
Construct Design
We obtained the amino acid sequence of the SplintR ligase protein found in Paramecium bursaria Chlorella virus 1. We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site. We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 790
- 1000COMPATIBLE WITH RFC[1000]