Difference between revisions of "Part:BBa K3352000"
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<partinfo>BBa_K3352000 short</partinfo>
 
<partinfo>BBa_K3352000 short</partinfo>
  
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*Construct Design*
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Construct Design
 
We obtained the amino acid sequence of the SplintR ligase protein found in Paramecium bursaria Chlorella virus 1. We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site. We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker).  
 
We obtained the amino acid sequence of the SplintR ligase protein found in Paramecium bursaria Chlorella virus 1. We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site. We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker).  
  

Revision as of 05:02, 18 October 2020


SplintR Ligase with His-Tag and GS Linker Sequence

Construct Design We obtained the amino acid sequence of the SplintR ligase protein found in Paramecium bursaria Chlorella virus 1. We optimized the DNA sequence for expression in E. coli and removed the PstI cutting site. We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
  • 1000
    COMPATIBLE WITH RFC[1000]