Difference between revisions of "Part:BBa K3431050"
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The is a sfGFP (BBa_K3431031) generating device regulated by Banana Toehold Switch (BBa_K3431039), with T7 Promoter (BBa_I719005) and T7 Terminator (BBa_K731721) as recognition sites for T7 RNA Polymerase in PURExpress in vitro protein synthesis kit. | The is a sfGFP (BBa_K3431031) generating device regulated by Banana Toehold Switch (BBa_K3431039), with T7 Promoter (BBa_I719005) and T7 Terminator (BBa_K731721) as recognition sites for T7 RNA Polymerase in PURExpress in vitro protein synthesis kit. | ||
| + | |||
| + | ===Experiment result=== | ||
| + | <html> | ||
| + | <div style="width=100%; display:flex; align-items: center; justify-content: center"> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/f/fc/T--CSMU_Taiwan--Fig._2_%28EXP_1%29.png" style="width:100%"> | ||
| + | </div> | ||
| + | <br> | ||
| + | </html> | ||
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Revision as of 17:56, 23 October 2020
Banana_ToeholdSwitch-Regulated sfGFP
The is a sfGFP (BBa_K3431031) generating device regulated by Banana Toehold Switch (BBa_K3431039), with T7 Promoter (BBa_I719005) and T7 Terminator (BBa_K731721) as recognition sites for T7 RNA Polymerase in PURExpress in vitro protein synthesis kit.
Experiment result
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 865
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]