Difference between revisions of "Part:BBa K3332017"
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This is an anchored proteins onto membranes through Lpp-OmpA and use GFP to comformation. We use K880005 and GFP to verify Lpp-OmpA's function which can anchor proteins onto membranes.
 
This is an anchored proteins onto membranes through Lpp-OmpA and use GFP to comformation. We use K880005 and GFP to verify Lpp-OmpA's function which can anchor proteins onto membranes.
  
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===Biology===
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===Usage and Biology===
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lpp-OmpA is an anchor protein from ''E. coli'', which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display.
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EGFP is green fluorescent protein from jellyfish ''Aequorea Victoria'', which has been widely used as reporter for decades. EGFP is fused at N terminal with Lpp-OmpA so that EGFP may be displayed on the surface of E. coli.<ref>https://parts.igem.org/Part:BBa_E0420</ref><ref>http://2016.igem.org/Team:TJUSLS_China</ref>
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===Usage===
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Here, we used <partinfo>BBa_K880005</partinfo> to construct the expression system and obtained the composite part <partinfo>BBa_K3332062</partinfo>. Due to the limited time, we didn’t get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.
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<html>
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    <figure>
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        <img src="https://2020.igem.org/wiki/images/8/88/T--XMU-China--XMU-China_2020-J23100_B0034_Anchor_with_EGFP_fused_B0015.png" width="45%" style="float:center">
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        <figcaption>
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        <p style="font-size:1rem">
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        </p>
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        </figcaption>
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    </figure>
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</html>
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Fig 1. Gene circuit of Lpp-OmpA-EGFP
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===References===
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<references/>
  
 
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Revision as of 20:24, 26 October 2020


Lpp-OmpA-GFP

This is an anchored proteins onto membranes through Lpp-OmpA and use GFP to comformation. We use K880005 and GFP to verify Lpp-OmpA's function which can anchor proteins onto membranes.

Biology

        Lpp-OmpA is an anchor protein from E. coli, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display.

        EGFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. EGFP is fused at N terminal with Lpp-OmpA so that EGFP may be displayed on the surface of E. coli.[1][2]

Usage

        Here, we used BBa_K880005 to construct the expression system and obtained the composite part BBa_K3332062. Due to the limited time, we didn’t get the gene in time. As a result, there is a lack of data about this part. The progress of this part remains in the stage of design.

Fig 1. Gene circuit of Lpp-OmpA-EGFP

References

  1. ↑ https://parts.igem.org/Part:BBa_E0420
  2. ↑ http://2016.igem.org/Team:TJUSLS_China

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109