Difference between revisions of "Part:BBa K3425130:Design"

 
(Added references, source and design notes)
 
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__NOTOC__
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<partinfo>BBa_K3425130 short</partinfo>
  
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<partinfo>BBa_K3425130 SequenceAndFeatures</partinfo>
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===Design Notes===
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When designing this part, we had to make sure that the binding site for NarL was included in the sequence.
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===Source===
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The sequence was obtained from the ''E. coli'' strain DH5α.
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===References===
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Lin, H.-Y., Bledsoe, P. J., and Stewart, V. (2007) Activation of yeaR-yoaG Operon Transcription by the Nitrate-Responsive Regulator NarL Is Independent of Oxygen- Responsive Regulator Fnr in Escherichia coli K-12. JB. 189, 7539–7548

Latest revision as of 22:07, 25 October 2020

YeaR Regulatory Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part, we had to make sure that the binding site for NarL was included in the sequence.

Source

The sequence was obtained from the E. coli strain DH5α.

References

Lin, H.-Y., Bledsoe, P. J., and Stewart, V. (2007) Activation of yeaR-yoaG Operon Transcription by the Nitrate-Responsive Regulator NarL Is Independent of Oxygen- Responsive Regulator Fnr in Escherichia coli K-12. JB. 189, 7539–7548