Difference between revisions of "Part:BBa K3264022:Experience"
(→Applications of BBa_K3264022) |
(→Pathway for BBa_K1131000) |
||
| Line 6: | Line 6: | ||
== Pathway for BBa_K1131000== | == Pathway for BBa_K1131000== | ||
Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed: <br> <br> | Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed: <br> <br> | ||
| − | [[File: | + | [[File:HBHB.png]] |
<!--Upon cloning this enzyme into E. coli with the [https://parts.igem.org/Part:BBa_J23100 BBa_J23100 Anderson Promoter], and adding tryptophan (a precursor to indole) in the LB media, we observed a dense blue color in our cultures. <br>[[File:blue tube.jpg | 350px]]--> | <!--Upon cloning this enzyme into E. coli with the [https://parts.igem.org/Part:BBa_J23100 BBa_J23100 Anderson Promoter], and adding tryptophan (a precursor to indole) in the LB media, we observed a dense blue color in our cultures. <br>[[File:blue tube.jpg | 350px]]--> | ||
Revision as of 15:25, 16 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Pathway for BBa_K1131000
Flaving Containing-Monooxygenase (FMO) is an enzyme that can be used to produce indigo. The enzyme is involved in the conversion of indole to indoxyl using NADPH as its co-factor. And upon exposure to oxygen, indoxyl spontaneously dimerzes to indigo. Specifically, this is the pathway being employed:
User Reviews
UNIQ3d5f458e9cb9ec3d-partinfo-00000000-QINU
|
No review score entered. SZU-China |
test test
|
UNIQ3d5f458e9cb9ec3d-partinfo-00000002-QINU