Difference between revisions of "Part:BBa K3505008"

 
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Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening.  
 
Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening.  
  
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===Usage and Biology===
 
===Usage and Biology===
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[[File:T--Thessaly--a1r.png|500px|thumb|left|<i><b>Fig.1:</b> a1R plasmid Features and Restiction Sites.</i>]]
  
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[[File:T--Thessaly--a1r_map.png|500px|thumb|left|<i><b>Fig.2:</b> a1R Map.</i>]]
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K3505008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3505008 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K3505008 parameters</partinfo>
 
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Revision as of 09:37, 23 October 2020


pDGB1 alpha 1R (α1R) for Bacterial GoldenBraid.

Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening.


Usage and Biology

Fig.1: a1R plasmid Features and Restiction Sites.
Fig.2: a1R Map.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3091
    Illegal PstI site found at 3079
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3118
    Illegal PstI site found at 3079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3118
    Illegal BamHI site found at 11
    Illegal BamHI site found at 2858
    Illegal BamHI site found at 3097
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3091
    Illegal PstI site found at 3079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3118
    Illegal XbaI site found at 3091
    Illegal PstI site found at 3079
    Illegal NgoMIV site found at 1290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3461
    Illegal BsaI.rc site found at 2879