Difference between revisions of "Part:BBa K3570006"

Revision as of 14:02, 15 October 2020

DPP1 upstream homologous sequence

Usage

DPP1 upstream homology arm part shall be used together with DPP1 downstream homology arm part (BBa_K3570007) to target a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.

This sequence was identified from a personal communication with Dr. Gilles Truan.

Experiments

References

S. cerevisiae genome, chromosome IV, DPP1 gene. GenBank: CP046084.1

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 50
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 50
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 50
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 50
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 DPP1 upstream homology arm part shall be used together with DPP1 downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570007 BBa_K3570007]) to target a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae's</i> genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene. </p>
 <p style="text-indent: 40px">
-This sequence was identified from a personal communication from Dr. Gilles Truan. </p>
+This sequence was identified from a personal communication with Dr. Gilles Truan. </p>
 <h2>Experiments</h2>