Difference between revisions of "Part:BBa K3394001"

Revision as of 07:31, 15 October 2020

Coding Sequence of CBD (Cellulose Binding Domain)

Most cellulolytic complexes consist of catalytic domains (Cellulases) and Cellulose-Binding Domains (CBDs).

The binding domain improves the attachment of the enzyme to the substrate and facilitate the catalytic domain activity. Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.

Figure 1: CBD - Cellulose-Binding Domains

The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 37
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 37
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 37
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 37
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 37
Illegal AgeI site found at 12
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Most cellulolytic complexes consist of catalytic domains (Cellulases) and Cellulose-Binding Domains (CBDs).
-[[File:CBD.jpg]]
-[[File:T--BGU-Israel--CBDimage.jpg]]
 The binding domain improves the attachment of the enzyme to the substrate and facilitate the catalytic domain activity.
 Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.
+[[File:T--BGU-Israel--CBDimage.jpeg]]
+[[File:T--BGU-Israel--CBDimage.jpeg|200px|thumb|left|Figure 1: CBD - Cellulose-Binding Domains]]
 The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.
 <!-- Add more about the biology of this part here
-[[File:File:BBa_K3394001-CBD_image.png]]
-[[File:BBa_K3394001-CBD_image.png|200px|thumb|left|Figure 1: CBD - Cellulose-Binding Domains]]
 ===Usage and Biology===