Difference between revisions of "Part:BBa K3585007"
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Combining the butyrate synthesis pathway enzyme and LacY protein, the engineered strain can effectively degrade galactose in the presence of glucose. | Combining the butyrate synthesis pathway enzyme and LacY protein, the engineered strain can effectively degrade galactose in the presence of glucose. | ||
| + | [[File:T--Shanghai_HS_United-BBa_K3585007 fig 00.png|400px|thumb|center|]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 14:03, 27 October 2020
pJ23100-butyrate-J23200-lacY
In E. coli, the presence of glucose inhibits the synthesis of many catabolic enzymes, a phenomenon called "glucose effect" or "glucose inhibition". Studies have shown that overexpression of LacY can eliminate the glucose effect. We use the strong promoter J23200 to continuously overexpress LacY protein to achieve simultaneous metabolism of glucose and galactose. Continuous expression of butyrate synthesis pathway enzymes through a constitutive promoter, so the strain can degrade galactose and synthesize butyrate. Combining the butyrate synthesis pathway enzyme and LacY protein, the engineered strain can effectively degrade galactose in the presence of glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1240
Illegal NheI site found at 8195
Illegal NheI site found at 8218 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6272
Illegal BglII site found at 7408
Illegal BglII site found at 7549
Illegal XhoI site found at 1245 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 9008
- 1000COMPATIBLE WITH RFC[1000]