Difference between revisions of "Part:BBa K079017"
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This parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079045 Lac Operator Library]. | This parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079045 Lac Operator Library]. | ||
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[[Image:LacOperon.jpg |left|350px|thumbnail|Regulatory Elements position in natural Lac Operon ]] | [[Image:LacOperon.jpg |left|350px|thumbnail|Regulatory Elements position in natural Lac Operon ]] | ||
[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079018 K079018]: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence. | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079018 K079018]: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence. | ||
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079017 K079017]: SymL is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about '''10-fold''' tighter than the O1 natural sequence | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079017 K079017]: SymL is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about '''10-fold''' tighter than the O1 natural sequence | ||
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Revision as of 12:01, 28 October 2008
Lac symmetric - operator library member
This parts have been extracted from the Lac Operator Library.
K079018: Lac O1 is the strongest wild type operator sequence in Lac Operon. In the natural system is located right after the -10 consensus sequence.
K079019: LacO2 is the intermediate affinity operator site, located 400 bp downstream of O1. In natural Lac Operon is responsible for Dna Looping, enanching repression and cooperativity of Lac system.
K079017: SymL is a perfectly symmetric Lac operator synthetically designed to maximize operator-repressor interaction. Its binding is about 10-fold tighter than the O1 natural sequence
This subset of operator sequences has been chosen since it allows to cover a wide affinity and repression range
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]