Difference between revisions of "Part:BBa K3610032:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | With the native signal peptide removed, the receptor sequence cannot be tranlated in-frame from the start. The scar "ggCAC" between the singal peptide and the receptor encoding domain is important for correct translation. | |
===Source=== | ===Source=== | ||
Revision as of 11:34, 14 October 2020
BAK1 ectodomain / YFP
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 867
Illegal PstI site found at 912 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 867
Illegal PstI site found at 912 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 867
Illegal PstI site found at 912 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 867
Illegal PstI site found at 912 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
With the native signal peptide removed, the receptor sequence cannot be tranlated in-frame from the start. The scar "ggCAC" between the singal peptide and the receptor encoding domain is important for correct translation.
Source
BBa_K319005, BBa_J63003, BBa_K2809002, BBa_K3610003, BBa_K416001, BBa_J176006, BBa_K2753050
References
Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.
Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.
Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.