Difference between revisions of "Part:BBa K3629022"
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===Usage and Biology=== | ===Usage and Biology=== | ||
mCitrine is a monomeric green fluorescent protein that is derived from <i>Aequorea victoria.</i> mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm. mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. | mCitrine is a monomeric green fluorescent protein that is derived from <i>Aequorea victoria.</i> mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm. mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. | ||
| + | See [https://parts.igem.org/Part:BBa_K3629026 BBa_K3629026] for a functional high expression construct using this coding sequence. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 10:57, 25 October 2020
mCitrine codon optimized for Yarrowia lipolytica
mCitrine coding sequence codon-optimized for Yarrowia lipolytica.
Usage and Biology
mCitrine is a monomeric green fluorescent protein that is derived from Aequorea victoria. mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm. mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. See BBa_K3629026 for a functional high expression construct using this coding sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 682
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 647