Difference between revisions of "Part:BBa K3629023"

 
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<partinfo>BBa_K3629023 short</partinfo>
 
<partinfo>BBa_K3629023 short</partinfo>
  
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LEU4 expression construct with Gibson homology, TEF promoter and double XPR2 terminator, and gPCR primer for Yarrowia lipolytica
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2-isopropylmalate synthase (LEU4) expression construct with Gibson homology, TEF intronic promoter (BBa_K3629002) double XPR2 terminator (BBa_K3629005), <i>Yarrowia lipolytica</i> LEU4 coding sequence (BBa_K3629019) and reverse gPCR primer binding site for <i>Yarrowia lipolytica.</i>
  
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__TOC__
 
===Usage and Biology===
 
===Usage and Biology===
  
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===Design===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K3629023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3629023 SequenceAndFeatures</partinfo>
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===References===
  
  

Revision as of 02:05, 14 October 2020


2-isopropylmalate synthase (LEU4) overexpression construct with Gibson homology

2-isopropylmalate synthase (LEU4) expression construct with Gibson homology, TEF intronic promoter (BBa_K3629002) double XPR2 terminator (BBa_K3629005), Yarrowia lipolytica LEU4 coding sequence (BBa_K3629019) and reverse gPCR primer binding site for Yarrowia lipolytica.

Usage and Biology

Design

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 339
    Illegal EcoRI site found at 2875
    Illegal SpeI site found at 569
    Illegal PstI site found at 534
    Illegal PstI site found at 986
    Illegal PstI site found at 2144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 339
    Illegal EcoRI site found at 2875
    Illegal NheI site found at 91
    Illegal SpeI site found at 569
    Illegal PstI site found at 534
    Illegal PstI site found at 986
    Illegal PstI site found at 2144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 339
    Illegal EcoRI site found at 2875
    Illegal BglII site found at 1629
    Illegal XhoI site found at 1329
    Illegal XhoI site found at 1878
    Illegal XhoI site found at 2136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 339
    Illegal EcoRI site found at 2875
    Illegal SpeI site found at 569
    Illegal PstI site found at 534
    Illegal PstI site found at 986
    Illegal PstI site found at 2144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 339
    Illegal EcoRI site found at 2875
    Illegal SpeI site found at 569
    Illegal PstI site found at 534
    Illegal PstI site found at 986
    Illegal PstI site found at 2144
  • 1000
    COMPATIBLE WITH RFC[1000]

References