Difference between revisions of "Part:BBa K3425033:Design"
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+ | For aligned spacing refer: Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745 |
Revision as of 10:16, 14 October 2020
iGEM Type IIS pSB1K03-DY
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Spacer provides optimal distance for E. coli when Type IIS iGEM Standard assembly is implemented.
Source
iGEM Registry of Standard Parts BBa_B0030
References
For aligned spacing refer: Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745