Difference between revisions of "Part:BBa K3425031:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Spacer provides optimal distance for E. coli when Type IIS iGEM Standard assembly is implemented.
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The spacer provides aligned spacing (the distance between RBS and ATG) of 9 nucleotides when Type IIS iGEM Standard assembly is used.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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For <b>aligned spacing</b> refer:
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Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745

Revision as of 10:26, 14 October 2020


iGEM Type IIS pSB3C11-DY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacer provides aligned spacing (the distance between RBS and ATG) of 9 nucleotides when Type IIS iGEM Standard assembly is used.

Source

iGEM Registry of Standard Parts BBa_B0032

References

For aligned spacing refer:

Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745